Using Honey in Inhibiting Staphylococcus aureus
ANBAR JOURNAL OF AGRICULTURAL SCIENCES,
2013, Volume 11, Issue 2, Pages 410-418
AbstractTwenty eight samples from individuals at the Burning Ward in Ramadi General Hospital were collected. The objective was to isolate Staphylococcus aureus. These samples were all taken from patient women.
Results have shown all samples belong to Staphylococcus, and that (26) isolates belong to Staphylococcus aureus germ with a ratio of (29.9%) and (2) isolate belong to Staphylococcus epidermidis with a ratio of (7.1%). Germ isolates were diagnosed using morphological and biochemical characteristics. The sensitivity of the isolates were tested against seven types of antibiotics. It was found that the highest average of inhibition diameter was (32) mm for Ciprofloxacin followed by Chromphenicol with an inhibition diameter of (30) mm, whereas Rifampin, Tobramycin and Cephotaxime gave the lowest inhibition diameter which was (2) mm.
After that different concentrations ofnormal honey (20%, 30%, 40%, 50%, 60%, 70%, 80% besides the reference 100% concentration) were prepared. The inhibition activity of each of these concentrations on the different isolates of Staphylococcus using the spread method was carried out in addition to comparing the concentration inhibition circle with the used antibiotics. It was observed that the inhibition activity of the eight concentrations varied with concentration.
The reference concentration gave a significant supremacy over all types of concentrations in inhibiting bacteria growth with an average of (42) mm Results of inhibition diameter of honey concentrations were compared with antibiotics. The highest inhibition activity belonged to honey concentration of 100% which was (42) mm representing inhibition activity higher than all antibiotics.
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