A study was conducted at college of Agriculture University Anbar for evaluate the suitability of available sulfur water resources in the western desert region ,The study was focused on the available water treatment choices which guaranteed using sulfur water without any environment or health risks. A field study and survey for the existing wells was carried out including determination at wells flow rate, sulfur compounds content. Abundance and distribution for Sulfur oxidizing and reducing bacteria was isolated and study. The results showed the sulfur water was already existing in the western desert region particulate in the kilo 70 area which has the highest sulfur content, four sulfur oxidizer bacterial isolates were obtained 40% percentage and identification 2 isolates the first on T. thiopurus and the second T. dovellus, also gets 2 isolates from the sulfur reductase anaerobic bacteria with 20% percentage , identification to Desulfovibro spp. also microbial inoculated was prepared.