Isolation of a dapttable or tolerant rhizobium and test its growth and survival in different salt concentration
ANBAR JOURNAL OF AGRICULTURAL SCIENCES,
2009, Volume 7, Issue 4, Pages 290-296
AbstractThe objective of this study was to test the ability of thirty one Rhizobium isolates which selected from the root nodules of peanut, lup, vicia fab, mumber and alfalfa to surive in four concentration of sodium chloride in teast lead tract mamnitol broth 5%, 10%, 15% and 25%. The study also include the ability of four local Rhizobium isolates to growth and survive in YEM with three sodium chloride concentration 0.002% (control), 1%, 5% for five period, zero, 1, 2, 3, 5 and 7 day after incubation. The results showed that these isolates differ in their tolerance to the different concentration of NaCl which adoled to YEM belonging to the host plants. Generally the viable cells of these four Rhizobium isolates decrease with the increasing of NaCl to 1% and 5% in broth media.
The results showed that the effect of time of incubation depend on the properties of the isolate for expnple local isolate number (1) showed the hight tolerant to 25% concentration of NaCl on broth media while it affected more with the increasing of incubation time and it differed with the local isolate number 23 which was less affected with incubation time and this may due to the difference in physiological properties of these isolates and its ability to adopt to the height salt concentration.
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