Study of Optimum Condition for Production of Glutamic Acid from Corynebacterium glutamicum HM16
ANBAR JOURNAL OF AGRICULTURAL SCIENCES,
2005, Volume 3, Issue 1, Pages 223-237
AbstractOptimum conditions for production of glutamic acid from Corynebacterium glutamicum HM16 isolated from soil were studied. It was found that glucose and ammonium sulphate were the best carbon and nitrogen sources in percentage of 7.0 and 2.3%, respectively. Yeast extract and peptone were found to be best organic enrichment substances when used in concentration of 0.2% for each of them. Manganese as MnO4.4H2O was necessary to increase the productivity, while iron had no detectable effect on production of glutamic acid.
The optimum conditions of production of glutamic acid from the bacterium were temperature 30C, pH 7.0, shaker velocity 150 rpm and inoculum concentration 4106 colony – forming unit (cfu)/ml of production medium in a period of 48 hr. The bacterium intered the stationary phase in 36 hr. About 89.4% of glucose was consumed during the priod of production of glutamic acid. The pH of the medium dropped to 6.0. Glutamic acid productivity was 9.6 mg/ml of production medium, which represented 68.1% of total amino acids.
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