ANBAR JOURNAL OF AGRICULTURAL SCIENCES

Abstract

The study was conducted on black mustard Brassica nigra seeds.The Percentage rate of seed component were2% moisture, 23.30% Protein ,35.88% fat ,5.52% ash , 5.00% fiber and 28.30% carbohydrates. The preliminary chemical analysis of active compounds showed that seeds contain glycosides, alkaloids, resin, flavonoids , phenol and saponines. Each aqueous and alcohols extracts were tested for antimicrobial activity towards (7) different strains of microorganisms included (3) bacterial which were Escherishia coli , Pseudomonas aeruginosa and Staphlyococcus aureus as well as 4 species of molds which were Alternaria alternata , Trichoderma harzianum , Penecillium italicum , Aspergillius niger . The inhibitory activity of extracts were varying and based on method of extraction and microorganism. The water extracts had shown clear inhibition towards bacterial strain E. coli and P. Aeruginosa . The alcoholic extract had low inhibition activity against the bacterial strains compared with water extracts, The water extracts at (50,75,100 )% were not showed any inhibition effect toward A. niger The alcoholic extracts was more effect on P. italicum , percentage of inhibition was 75.5% than another molds which were A . alternata , T. harzianum , A. niger that their percentage inhibition were (62.0 , 38.8, 20.22) % respectively.