This study aimed to evaluate the effect supplementation Nigella sativa , Artemisia annua and their mixture on some hematological, immunological and biochemical characteristics on broiler chicks. one hundred thirty five unsexed broiler chicks Ross breed of one day old have been used in this study.The chicks were distributed into 7 groups randomly. T1 was regarded as the control group that given an basic ration without any additive. T2 supplementation 0.25% Nigella sativa,T3 supplementation 0.5% Nigella sativa, T4 supplementation 0.25% Artemisia annua, T5 0. 5% Artemisia annua T6 0.25% Nigella sativa + Artemisia annua T7 0.5% Nigella sativa + Artemisia annua. The result showed a significant increase (P<0.05) hemoglobulin in all groups as compared with control group except T4 and T5 Also study showed a significant increase (P<0.05) of Packed volume cell in all groups as compared with control group except T5 while the highest degree 29.55 in T3 compared with control group 25.48. All treatment showed significant increase on in serum total protein except T3. Also the globulin concentration showed significant increase for treatments group T5,T6 (P<0.05) compared with all treatments and showed same group a significant decrease (P<0.05) in cholesterol in compared with control treatment.