Extraction and Purification of Cellulase from Ruminants Rumen Liquor
ANBAR JOURNAL OF AGRICULTURAL SCIENCES,
2011, Volume 9, Issue 3, Pages 0-0
AbstractProducing cellulase by rumen cellulytic microorganisms was extracted and purified by dialysis and DEAE-Sephadex A50 Ion-exchange chromatography 8.53 fold with a 40.02 % yield and a specific activity of 718.4 U/mg. It was optimally active at pH 6 and 40 °C while stable from pH 5 to 7 and 20 to 40 °C. A 3, 5 - Dinitro Salicylic Acid (DNSA) solution was used for measuring cellulase activity at 575 nm.
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