The research was conducted by using the ethanolic extract and powder of Eucalyptus leeves to evaluate their effect on some biological aspects of potato tuber moth in the laboratory . Results showed the important role of Eucalyptus extracts on potato tuber moth , the ethanolic extract of Eucalypt leeves prolonged the period of egg development which increased to 7.33 days by using 10000 ppm while the egg hatching percentage was decreased to 71 % at the same dose of extract , Adult emergence was decreased to74.67% and females fecundity decreased to 57.33 egg. Pupae treated with 10 % powder resulted in prolonged development period which reached to 15.33 days . Potato tubers treted with extract decreased number of egg on tubers by females to10.33 egg at 10000 ppm . There was also a clear effect for the ethanolic extract in larvae growth , percentage of number of first instar larva decreased by 91.3 % , and third instar larva decreased by 61 % when using 10000 ppm of ethanolic extract . These results indicated the possibility of using the natural materials of plants which are safe, cheep and active for pest control .