The present research paper has been carried out to isolate and determine urea degenerating and urease producing bacteria from different sources including agriculture soils, animal yards, river water and health drainage from varies locations in Anbar province and patients divresis samples of Ramad Educational Hospital. Also, it tries to study the effect of temperatures,(CaCO3) ,(CaSO4) and Nickel on the rate of the activity of urea degenerating bacteria, Isolates producing Urease enzyme have been identified as belonging to urea degenerating bacteria with a dominant rate of 58.3% in all isolates. Five bacterial isolates have been elected as the most efficient in urea degenerating, namely; Bacillus KS4, Ochrobactor anthropi RM30, Raoultella planticola WR43, Enterobacter cloacae Rw47 and Proteus mirabilis U70. Results have shown that the best activity of urea degenerating and Urease producing bacteria was at 35 and 40 c˚ temperatures. The activity decreased when incubated at 15 and 50 c˚ temperatures and stopped when incubated at 60 c˚. The activity rate of isolates increased when increasing the percentage of gypsum in the medium to 4 and 5%, while the activity in degenerating urea compound decreased when using increasing amounts of gypsum above 1%. When using 5% of gypsum, the activity of isolates severely decreased. The use of 0.01% of Ni in the medium has led to increasing the isolates activity,whereas their activity decreased at 0.02% of Ni.