A lath tria was carried out at lathhouse belonging to the Department of Horticulture and Landscaping, College of Agriculture, University of Anbar for the year 2020-2021. For the purpose to test the effect of spraying with nano-proline and marine algae extract on rosemary plant, three levels of nano-proline were used viz., 0, 200 and 400 mg L-1. And three levels of seaweed extract 0, 100 and 200 mg L -1 a significant and clear effect of spraying nano-proline was recorded on the studied traits compared to plants that were not sprayed, a significant increase was also recorded in the studied traits. It was found that treatment with Proline at the highest concentration 400 led to a significant increase in all these studied traits (1.7956%), (12.6733) mg/100g, and (13.7800%), respectively. The results showed that treatment with nanorolene led to a significant increase in the leaves content of nutrients (N.P.K) significantly at concentration 400. which were sprayed with marine algae extract, which is distinguished by the interaction between the two factors of the study, where the highest rates of the studied traits were recorded in the combination 400 Proline + 200 Seaweed extract. A significant increase was also recorded in the studied traits, which were sprayed with marine algae extract, the treatment with marine algae extract positively affected the chemical characteristics, as the concentration 200 mg. plant-1 gave the highest values for these traits. (2.1433% for carbohydrates), (14.33 for chlorophyll), (14.022% for the percentage of dry matter) and also had a significant effect on the percentage of nutrients in the plant and the values of the elements were (1.0137N: %) (0.03556P=%) (1.4033K=%) The interaction between the two factors of the study had a positive effect in all the studied traits, as the highest rates were recorded for the studied traits when the combination 400 P and 200A. Where the highest values were recorded compared to the rest of the treatments, including the measurement treatments, where the percentage of carbohydrates was 3.3967%, the content of leaves was chlorophyll 15.7567 and the percentage of dry matter 15.0733%. The results also show the clear superiority in the percentage of nutrients in the leaves, the value of which was N 1.1697%, P 1.427% and K 1.4933%.