Department of Animal Production, College of Agriculture, University of Anbar
Document Type : Research Paper
This experiment was conducted in the sheep farm of Department of Animal Production, College of Agriculture, University of Anbar, during the period of 17/10/2021 to 9/1/2022. Sixteen local male lambs were chosen between 5-6 months of age and 35.31 ± 3.72 kg of average body weight. The aim of the experiment was to determine the effect of melatonin implants and nutritional restriction on some blood biochemical parameters of local male lambs. The experimental period lasted for 69 days, the experiment involved two phases, feed restriction phase (42 days), and realimentation phase (27 days). Lambs were randomly distributed into four equal groups with four lambs of each. During the restriction phase, first group (T1) was used as a control and feed ad libitum. The second group (T2) was feed ad libitum and treated with two implants of melatonin (36mg) subcutaneously at the base of the ear. The third group (T3) was feed restriction 75% of ad libitum intake. The fourth group (T4) was feed restriction 75% of ad libitum intake and treated with two implants of melatonin subcutaneously at the base of the ear. Blood samples were collected from all treated groups at day 0, 42 and 69 for the measurement the level of glucose, total protein, triglyceride, aspartate aminotransferase (AST), alanine transaminase (ALT) and malondialdehyde (MDA) in the plasma. The results restricted feeding, with or without melatonin, did not affect significantly on blood biochemical parameters, while it was observed that the restricted feeding with or without melatonin significantly reduced the level of malondialdehyde. It was concluded from this experiment that feeding local male lambs at a level of 75% of ad libitum with or without melatonin implants for 42 days followed by realimentation for 27 days improved oxidative status in male lambs.
- Abecia, J. A., Luis, S., and Canto, F. (2021). Implanting melatonin at lambing enhances lamb growth and maintains high fat content in milk. Veterinary Research Communications, 45(4): 181-188.
- Abouheif, M., Al-Sornokh, H., Swelum, A., Yaqoob, H., and Al-Owaimer, A. (2015). Effect of different feed restriction regimens on lamb performance and carcass traits. Revista Brasileira de Zootecnia, 44(3):76-82.
- Abouheif, M., Al-Owaimer, A., Kraidees, M., Metwally, H. and Shafey, T. (2013). Effect of restricted feeding and realimentation on feed performance and carcass characteristics of growing lambs. Revista Brasileira de Zootecnia, 42 (2): 95-101.
- Abouheif, M., Al-Sornokh, H., Swelum, A., Shafey, T., Mahmoud, A., Alshamiry, F. and Haroon, R. (2016). Effects of intake restriction and realimentation on diet digestion and ruminal fermentation by growing lambs. Global Advanced Research Journal of Agricultural Science, 4(5): 126-131.
- Al-Jassim, A. F. H., Al-Hashimi, H. H. I., and Al-Bidhani, M. K. J. (2018). Effect of feed restriction with or without addition saccharomyces cerevisiae on blood and serum biochemical parameters of Arabian lambs. International Journal of Pure and Applied Bioscience, 6 (1): 1315-1321.
- Al-Ugaili, M. I. N. (2021). Effect of melatonin treatment with or without equine chorionic gonadotropin on reproductive performance of local Iraqi ewes. MSc. Thesis, College of Agriculture, University of Anbar.
- Amin, M. A. (2006). Influence of circadian rhythm on the physical and mental performance. MSc. Thesis, Louisiana State University.
- Babu, S. B., Suryanarayana, M. V. A. N., Rao, E. R., and Latha, P. A. (2017). Effect of feed restriction on performance and nutrient digestibility in ram lambs. International Journal of Current Microbiology and Applied Sciences,6(9): 566-572.
- Choudhary, P. K., Ishwar, A. K., Kumar, R., Niyogi, D., and Kumar, M. (2018). Effect of exogenous melatonin and different photoperiods on oxidative status and antioxidant enzyme activity in Chhotanagpuri. Veterinary World, 11(2): 130- 134.
- Cui, K., Wang, B., Ma, T., Si, B. W., Zhang, N. F., Tu, Y. and Diao, Q. Y. (2018). Effects of dietary protein restriction followed by realimentation on growth performance and liver transcriptome alteration of lamb. Scientific Reports,8(15185):1-10.
- Duncan, D. B. (1955). Multiple range and multiple F tests. Biometrics, 11: 1- 42.
- Ellis, N. (1980). The nutrient composition of Sudanese animal feeds. Bulletin No. 1. Northern and Central Sudan. Animal Nutrition Research Laboratory, Kuku, Khartoum North. Appendix 1, p. 19.
- Haldar, K. C. (2012). Correlation between peripheral melatonin and general immune status of domestic goat, Capra hircus: A seasonal and sex dependent variation. Small Ruminant Research, 107(2- 3): 147- 156.
- Legan, S. J., and Winans, S. S. (1981). The photoneuroendocrine control of seasonal breeding in the ewe. General and Comparative Endocrinology, 45(3): 317- 328.
- Mansoor, A. R. (2015). Study of melatonin levels and effect of implants on physiological, reproductive and productive performance on local ewes. Ph.D. Thesis, College of Agriculture, University of Anbar.
- Sainz, R. D. (1995). Why does feed restriction improve efficiency? In: Symposium: Intake by Feedlot Cattle, Oklahoma Agric. Exp. Sta., Stillwater, O.K., p. 175- 179.
- SAS. (2003). Version 9. 9th Ed. Cary, NC: SAS Institute.
- Tan, D. X., Chen, L. D., Poeggeler, B., Manchester, L. C., and Reiter, R. J. (1993). Melatonin: a potent, endogenous hydroxyl radical scavenger. Endocrine Journal, 1: 57- 60.
- Vázquez, M. I., Forcada, F., Sosa, C., Casao, A., Sartore, I., Fernández-Foren, A., Meikle, A., and Abecia, J. A. (2013). Effect of exogenous melatonin on embryo viability and uterine environment in undernourished ewes. Animal Reproduction Science, 141(1-2): 52- 61.
- Widiyono, I., Sarmin, and Putro, P. P. (2016). Influence of feed intake on blood chemistry parameters in Kacang goats. AIP Conference Proceedings1755,140011: 1- 5.
- Zhan, X. A., Wang, M., Zhao, R. Q., Li, W. F., and Xu, Z. R. (2007). Effect of different selenium source on selenium distribution, loin quality and antioxidant status in finishing pigs. Animal Feed Science and Technology, 132(3-4): 202-211.