A field experiment was carried out in the experimental field of Department of field crops- Agriculture College-University Of Anbar (Abu-Ghariab) during the two winter seasons of 2015/2016 and 2016/2017, to study the effect of cultivars and foliar application of amino acids at different concentrations in yield and quality of wheat crop. Randomized complete block design (R.C.B.D) was used with the arrangement of split-split plots with three replications. The main plots were occupied with amino acids (Tryptophan, Arginine and Tyrosine), while the sub-plots were occupied with amino acids concentrations (0, 100 and 200 mg L-1) and the sub-sub-plots were occupied with genotypes (Al-Rasheed, Tamooz-2 and Abu-Gharaib-3). Results of this study showed superiority of Al-Rasheed cultivars in period of grain filling (28.46 and 32.52 days) highest yield (6.32 and 7.09 ton ha-1), highest percentage of protein (13.81 and 14.41%), moist gluten ratio (36.27 and 38.21%) and carbohydrates percentage (70.42 and 68.62%) for both seasons respectively. As for amino acids concentrations, they significantly affected in studied traits and for both seasons. The concentration of 200 mg L-1 was superior with highest mean of protein percentage (13.86 and 14.49%) moist gluten and carbohydrates (71.00 and 69.20%) in both seasons respectively. Amino acids were varied significantly in The number of days of 100% flowering to physiological maturity, seed yield, protein percentage, moist gluten, carbohydrates, and amino acids content in seeds. Foliar application with Tyrosine gave highest mean of seed yield in both seasons (5.92 and 6.83 ton ha-1) and highest mean of gluten percentage in seed in first season (34.86%).