This study was conducted in the olive orchard, in Habbaniyah city, Anbar province 84 km west of Baghdad during 2018 growing seasons to investigate the influence of brassinolide (BRs) and CPPU spray on 2 year's old trees of “Nebali” olive cultivar. This study included two treatments: three levels of spraying of BRs, 0 (BR0), 1mg L-1 (BR1) and 2mg L-1 (BR2) and four levels of spraying of cytokinins (CPPU), 0 (C0), 2.5mg L-1 (C2.5), 5mg L-1 (C5) and 10mg L-1 (C10) and their interaction. Treatments were replicated three times with two transplants in experimental unit at factorial experiment in a RCBD. The number of trees used was 72 trees. The experimental results showed that BRs spray at 2 mg L-1 gave the highest leaves chlorophyll content of 62.69 SPAD units, leaves dry weight of 29.49 %, highest leaf area of 6.80 cm2, leaf nitrogen content of 1.169 %, highest leaf IAA content of 44.49 ?g g-1 FW and highest leaf GA content of 150.24 ?g g-1 FW. Results also showed that CPPU spray at levels 10 mg L-1 superiority of the control treatment and gave the highest leaves chlorophyll content of 62.89 SPAD units, leaves dry weight of 28.92 %, leaf IAA content of 41.82 ?g g-1 FW and highest leaf GA content of 134.00 ?g g-1 FW .The lowest value of these parameters was found in the control (BR0C0).