This study was conducted on Agriculture college of Abu-Grab/ Baghdad as a replacement location of Anbar University during 2015 / 2016 season to study the effects of Date palm frond incubation 30 days after soaking in solution of 5% Urea or 3.3% Ammonium hydroxide before using it to preparing a compost, and its effect on mycelium growth rate on solid medium, matt on liquid medium and protein content, and to study complete over growth time on compost media. Agaricus bisporus 169 strain and its spawn obtained from Organic Farming Project Belonging to Agriculture Researches Office / Agriculture Ministry. Simple experiments according to completely Randomized Design (CRD) with 3 replicates and 5 experimental units for each treatment .Genstat program was used to analyses all data and means tested by Least Significant Difference at 0.05 level. Results showed that, all composts prepared by usage partial from Date palm frond soaked in NH4OH solution increased vegetative growth of mycelium . Treatments of 75% wheat straw + 25% date palm frond and 50% wheat straw + 50% date palm frond given faster growth rat were 2.6 and 2.56 cm per day, while treatments of 75% wheat straw + 25% date palm frond and 50% wheat straw + 50% date palm frond soaked in Urea solution increased 2.5 and 2.53 cm day-1 respectively and increased protein content significantly (P.L. 0.05) were 24.30% and 26.25% respectively, when control treatment given 2.0 cm day-1, 0.15 g dry weight of biomass and 18.75% protein content respectively. On other hand compost from 50% wheat straw + 50% date palm frond soaked in water before incubation, 75% wheat straw + 25% date palm frond without treatment, 50% wheat straw + 50% date palm frond and 70% wheat straw + 25% date palm frond soaked in NH4OH reduced period of mycelia overgrowth on compost to 30.66 , 31.0 , 31.66 and 32.0 days respectively , while the control recorded 47.0 days.