A full cross was carried out among six inbred lines of maize in the autumn season (2016) in one of the fields of the college of Agriculture, University of Anbar (alternative site-Abu Ghraib-Baghdad). In the spring season (2017) west of Iraq. The experiment included cultivating the seeds of inbreds lines and hybrids resulting from the crossing among them, which included 30 hybrid and reverse hybrids. The aim of the study was to evaluate the behavior of inbred lines and their diallel hybrid and reverse axes by molecular methods.
The results of the Molecular Analysis of RAPD (Randomly Amplified Polymorphic DNA) showed that the five prefixes were successful in diagnosing 32 specialized cutting sites spread within the genomes of the paternal and crossbreeding genomes, 26 of them polymorphic loci, The results of the ISSR (Inter Single Sequence Repeats) indicators showed that the five prefixes used were particularly successful when compared with RAPD indicators. There were 40 correlation sites, 39 of which were multiform and 97% of the polymorphisms. Cluster analysis results for replicated sites were very similar in both techniques used in the study. The genotypes were separated into three main groups according to the nearest neighbor method. The two breeds 1 and 2 were the most divergent, as they belonged to the two most heterogeneous groups on both ends of the hierarchical assembly.