Abstract

An isolate of Bacillus cereus MH12 isolated and identified in a previous study and found to be highly producer of alkaline protease during the primary and secondary screening processes, which were used. In this study optimal condition for production of the enzyme by submerged culture were determined of the enzyme by submerged culture. Effect of medium components such as carbon sources and their concentration, the source of nitrogen and the physical factors were investigated which included primary pH, incubation period, incubation temperature, inoculum size and agitation speed. It was found that optimum of enzyme production achieved by using of Galactose as a carbon source in a concentration of 1% and peptone as a nitrogen source in a concentration of 0.5% under pH 9.0 through incubation time 96 hours at 37 °C with the use of a inoculum 3 ml per 50 ml contains 107 cells per ml with speed agitation of 150 rpm per min. At the end of the optimization, the enzyme activity was 199.83 in comparison with 81.00 U per ml before optimization. Thus, the productivity of the enzyme Bacillus cereus MH12 was increased 146.70% or about one and half times through this study