This study was carried out at Poultry Researches Station – Livestock Department in the Agricultural Researches Directorate in Baghdad Province/ Abu Gharib from 28/12/2015 to 26/1/2016, The aim of the study is to find out an important chemical changes in fertilized and unfertilized eggs during storage and incubation, the eggs were used in experiment from a local laying hens flock of the same station. 360 fertilized egg and 360 un-fertilized egg had been used and distributed into four treatments, 180 eggs for each treatment. The eggs of each treatment had been labeled and weighted, then two groups of fertilized and un-fertilized eggs had been stored for 7 days while others two groups for 4 days on the temperature 15-18 and 70-75% relative humidity. The eggs of treatments had been carried into the hatchery and incubated on 37.8 (99.5 Fahrenheit) and 60-65% relative humidity.
The results display that the effect of store 4 and 7 days were not different significantly in the values of (pH) for eggs whites and yolks, the results of (FTIR) showed certain changes in the minor formation of Protein in the fertilized egg yolks and whites in processing of store period because of protein change into the form which could be utilized by living creature.