This study was carried out at Poultry Researches Station – Livestock Department in the Agricultural Researches Directorate in Baghdad Province/ Abu Gharib from 28/12/2015 to 26/1/2016, The aim of the study is to find out an important chemical changes in fertilized and unfertilized eggs during storage and incubation, the eggs were used in experiment from a local laying hens flock of the same station. 360 fertilized eggs and 360 unfertilized eggs had been used and distributed into four treatments, 180 eggs for each treatment. The eggs of each treatment had been labeled and weighted, then two groups of fertilized and unfertilized eggs had been labeled and weighted, then two groups of fertilized and unfertilized eggs had been stored for 7 days while others two groups for 4 days on the temperature 15-18 and 70-75% relative humidity. The eggs of treatments had been carried into the hatchery and incubated on (37.8 99.5 Fahrenheit) and 60-65% relative humidity.
The results of experiment showed that the store period 4 and 7 days have not significant differences in the internal quality specifications of eggs because of low evaporation of water from the inside of egg into the external space, Also the results of (Electrophoresis) showed changes in the concentration of protein during the store period, the concentration of albumen proteins had lowered more than yolks protein in fertilized eggs.