Effect of reconstitution ratio on viability of Lactobacillus acidophilus and Bifidobacterium breve dried by vacuum oven
ANBAR JOURNAL OF AGRICULTURAL SCIENCES,
2017, Volume 15, Issue 0, Pages 523-530
AbstractReconstituted skim milk were used in 12, 20 and 25% to cultivate Lactobacillus acidophilus (Lb.a) and Bifidobacterium breve (Bifi.b) in 37° C for 24-48 hours after individually inoculation with 10% of bacteria. 20% of reconstitution was chosen for highest growth rate of bacteria among them, the logarithmic numbers of bacteria are 10.04 and 9.85 for Lb.a and Bifi.b respectively, whereas titratable acidity of milk were 0.72 and 0.65% respectively, The pH values of the 20% reconstitution milk were 4.6 and 4.9 respectively. Cultured Lb.a and Bifi.b bacteria were dried by using vacuum oven with or without protectants. The drying were affected on viability of lactic acid bacteria, drying in vacuum oven gave the best results when protectants material was added, logarithmic viable numbers were 9.26 and 9.04 for Lb.a and bifi.b respectively, followed by, Logarithmic viable count of dried cultures in vacuum oven before adding the protectants were 9.12 and 8.92 respectively. Moisture content were 4 and 4.10% respectively for Lb.a and bifi.b in dried cultures by using vacuum oven with protectants, while that were 4.23 and 4.26% respectively in dried cultures by vacuum oven without protectants.
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