Identification of the genetic diversity and physiological response of five inbred lines and their half diallel crosses under water stress
ANBAR JOURNAL OF AGRICULTURAL SCIENCES,
2017, Volume 15, Issue 0, Pages 95-113
AbstractA field experiment was conducted in research station of field crops department - Agriculture College- University of Anabr (Abu-Ghariab) during the spring season of 2016 in order to diagnose the genetic diversity using five random primers and assessment of physiological response for 5 maize inbred lines and their half diallel crosses under the effect of two irrigation intervals (each 5 and 10 days). A spilt plot arrangement was used in RCBD with three replications; irrigation intervals occupied the main plots while the genotypes occupied the sub plots.
The genotype were responding differently for all studied traits, the hybrid S4xS5 under 5 days irrigation interval was superior for plant dry weight by 441.40 g plant-1, and plant yield with 12.29 ton ha-1, while the hybrid S2xS5 was superior by achieving highest average of leaf area under 5 days irrigation interval with 765.75 cm2 plant-1, while the hybrid S1xS3 under 5 days irrigation interval gave the highest average of plant height by 204.20 cm. The hybrid S2xS4 was superior during the 5 days interval by giving highest average of number of grains per row with 41.80 grain row-1. All primers produced 65 bands and there were 13 divert bands when OPC-15 primer was used with molecular weight of 200-2500 bp, however the whole DNA segments of all primers were fell between 200 and 3000 bp. A unique band was determined in S5, S3xS4 and S3xS5 at OPC-15, OPD-03 and OPD-05 respectively. It can be concluded from those results that the extension of irrigation interval negatively affected the performance of genotype in field as well as there was a genetic diversity between some of the genotypes under study and it can be recommended to use some other primers to pick more difference between genotypes.
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