This study was conducted in the sheep farm of Department of Animal Production Sheep Field /Faculty of Agriculture University of Baghdad in the Jadiriyah area, as well as laboratories of Genetic Engineering and the Institute of Life Technologies Graduate Studies-University of Baghdad, during the period from 01/04/2015 to 01/02/2016, in order to identify genetic manifestations gene AA-NAT and its relationship with some of reproductive and productive traits among a sample of 30 Turkish Awassi ewes and offspring of 38 births. There was a genetic variation in polymorphism manifestations of the gene AA-NAT depending on the different genetic packets that generated by enzymatic digestion, which showed to be of three appearances (3 packs AA; 5 packs GA and 4 packs GG). The distribution of genetic manifestations attributed to gene AA-NAT in a sample Awassi sheep 56.67 and 33.33 and 10.00% for each of the genotypes AA and GA and GG respectively, and it was the contrast between these highly significant proportions, it was observed that the influence of appearance hereditary gene AA-NAT in the prolificacy and twining rate highly significant, while there was no significant difference in the fertility percentage. The results of the current study showed that the daily and total milk production of Awassi ewes was morally affected (P <0.05) genotype appearance, as ewes achieved with genotype AA the best rates, while those registered with genotype GG lowest rates. It can be concluded from this study of gene expression for gene AA-NAT possible adoption in the development of genetic improvement strategies for the sheep to maximize the economic returns of the election and crossing genotypes that have achieved the best prolificacy rate, twinning percentage and milk production.