Extraction of Peroxidase from Seeds of Some Plants belonging to Gramineae, Fabaceae and Solanaceae Families and Study of Biochemical Characteristics from the Selected Source
ANBAR JOURNAL OF AGRICULTURAL SCIENCES,
2016, Volume 14, Issue 1, Pages 314-326
AbstractThis study was conducted in order to extract peroxidase from seeds of 10 plants belonging to 3 families; Fabaceae (Mung bean, Lentils and Peanut), Solanaceae (Chili pepper, Bell pepper, Datura and wild eggplant),Poaceae (Oat, pearl millet and proso millet).the enzyme was qualified and its kinetic constants were assessed. Extract prepared from proso millet seeds using phosphate buffer 0.1M at pH7 contained the highest specific activity 3.35 U. mg.protein-1 as compared with seeds of other plants. In determination of optimum condition of enzyme extraction, three kinds of buffer solutions at two concentrations 0.1 and 0.2M within pH range 4.5-9 were used. Sodium acetate at pH 6 at 0.1M concentration gave the best results for enzyme extraction with specific activity of 8.06 U. mg.protein-1. The ratios of 1:4 recorded the best specific activity 5.98 U. mg.protein-1. Results showed that the optimum period of extraction was 30 min. with specific activity of 7.11 U. mg.protein-1. Precipitation with saturated solution of ammonium sulfate (30-90%) was used. the highest specific activity was noticed in saturation percent 80% and was 11.29 U. mg.protein-1 with purification fold of 1.66 and enzymatic yield of 70%.
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