In this study the optimum conditions to extract the mucilage from Tamarindus indica L. Seeds powder was determined. The extraction procedure was conducted at different temperature, mixing ratio of distilled water with seeds powder and seeds defatting as well. The results revealed that the temperature was found to be superior in mucilage yield. While defatting and mixing ratio was less effective. The extracted mucilage appeared in dark brown color at 100 C° in compared with other heat treatment. The highest yield of mucilage was achieved by extracting defatted seeds powder with distilled water using 1:40 (w/v) mixing ratio at 100 C° which resulted in 21.45% , While extracting the whole seeds powder and defatted seeds powder at 25 C° and 60 C° yielded 0.25 , 1.38 , 0.83 and 2.75% respectively .