Recording the first white mold disease Sclerotinia sclerotiorum on ornamental plants Alrannkil Ranunculus asiaticus in Iraq and effectiveness of the fungus Trichoderma viride and Pseudomonas aeruginosa bacteria in inhibition in vitro.
ANBAR JOURNAL OF AGRICULTURAL SCIENCES,
2015, Volume 13, Issue 2, Pages 326-338
AbstractShowed isolation results from plants Alrannkil planted in one of the greenhouses belonging to the Department of Horticulture - Faculty of Agriculture, University of Baghdad, which showed symptoms of yellowing and wilting leaves and rot loose down the stems of flowering and death of infected plants was accompanied by those symptoms, there are objects of stone small black color diameter 1 - 6 mm that the pathogen is a fungus Sclerotinia sclerotiorum. Pathogenicity tests have proven the ability to isolate the fungus S. sclerotiorum plants Alrannkil age of 30 days under the conditions of the plastic house plants Alrannkil by injury and severity of the injury was 100% and 87.5% on the relay. It was obtained isolation of the fungus Trichoderma viride as a result of insulation from stone-fungal objects and personalize this isolation based on Cultural and Morphological traits and molecular diagnosis of technology using PCR. Achieved isolation T. viride hottest against fungus S. sclerotiorum on culture media PDA under laboratory conditions has been the degree of antagonism 2 and the isolation of the bacteria Pseudomonas aeruginosa achieved over the center Zorai PDA ratio under laboratory conditions inhibition of 83.62%. The test showed the effect of each of the fungus Results T. viride and P. aeruginosa bacteria in the emergence of fruiting bodies of the stone objects of the fungus S. sclerotiorum in the laboratory when continuous lighting and a temperature of 15 ° C reduction was significant for the number of fruiting bodies formed conditions, amounting to 0.20 and 0.83 fruit body / stone body in relation to the treatment of comparative 1:58 body My fruit body / stone body.
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