Fourty-six different soil samples were collected from different geographic spaces at Baghdad , Babylon, AL-Anbar, Wast and Dylai govermorates to detectionn entomopathogenic ematodes by using of late larvae stages of greater wax moth. Results revealed that entomopathogenic nematode ,Steinernema spp.isolated from samples ;Wohda project(2)at Baghdad, Sadat AL-Hendia (2)at Babylon, AL-Lug (4)at Wast and AL-Obedi (1) at AL-Anbar while Heterorhabditis spp. isolated from samples Baguba (1) at Dylai and Tuwaiitha (3) at Baghdad .Laboratory study was conducted to evaluate efficiency of entomopathogenic nematode, 4 isolates of Steinernema spp. and two isolates Heterorhabditis spp. using concentrations of 0.0, 25, 50 infective nematode juvenile /5 larvaes of stem corn borer under laboratery conditions .Results obtained showed that all nematode concentrations used were efficient in infecting and causing high mortality of larvaes of stem corn borer. When used concentration 50 infective nematode juvenile of Heterorhabditis spp. caused the highest mortality (100%) after 2-3 days of treatment .