Keywords : Bacteria


Isolating Urease producing Bacteria from Different ecological Sources and Studying Some Factors Influencing its Activity

Aamal D. Abood; Idham A. Abed; Ahmed M. Turky

ANBAR JOURNAL OF AGRICULTURAL SCIENCES, 2012, Volume 10, Issue 1, Pages 185-197

The present research paper has been carried out to isolate and determine urea degenerating and urease producing bacteria from different sources including agriculture soils, animal yards, river water and health drainage from varies locations in Anbar province and patients divresis samples of Ramad Educational Hospital. Also, it tries to study the effect of temperatures,(CaCO3) ,(CaSO4) and Nickel on the rate of the activity of urea degenerating bacteria, Isolates producing Urease enzyme have been identified as belonging to urea degenerating bacteria with a dominant rate of 58.3% in all isolates. Five bacterial isolates have been elected as the most efficient in urea degenerating, namely; Bacillus KS4, Ochrobactor anthropi RM30, Raoultella planticola WR43, Enterobacter cloacae Rw47 and Proteus mirabilis U70. Results have shown that the best activity of urea degenerating and Urease producing bacteria was at 35 and 40 c˚ temperatures. The activity decreased when incubated at 15 and 50 c˚ temperatures and stopped when incubated at 60 c˚. The activity rate of isolates increased when increasing the percentage of gypsum in the medium to 4 and 5%, while the activity in degenerating urea compound decreased when using increasing amounts of gypsum above 1%. When using 5% of gypsum, the activity of isolates severely decreased. The use of 0.01% of Ni in the medium has led to increasing the isolates activity,whereas their activity decreased at 0.02% of Ni.

Studying the effect of organic solvent extracts for Euphorbia prostrata against bacteria Staphylococcus aureus and Pseudomonas aeruginosa in vitro

Rana Talib AL-Ani

ANBAR JOURNAL OF AGRICULTURAL SCIENCES, 2011, Volume 9, Issue 2, Pages 322-329

The aim of this study was to evaluate the biological activity of organic solvent extracts of Euphorbia prostrate on growth inhibition of Pseudomonas aeruginosa and Staphylococcus aureus and compare them with several antibiotics. The results of testing alcohol extracts showed high efficiency in inhibition of growth of S. aureus in concentration of 50mg/ml , but for P. aeruginosa , growth inhibition began at 70mg/ml. Results also showed that hexane extract had no effect on both bacteria. on the other hand, Ethyl acetate extract had inhibitive effects on S. aureus at 70mg/ml and on P. aeruginosa at 90mg/ml. When the activity of the three organic extracts on inhibition of test bacteria were compared with several antibiotics, identity of inhibition degree was found between them and even superiority on some others

Effect of using co- inoculums bacteria (Bradyrhizobium japonicum and Pseudomonas puteida ) to the improvement environment and Soybean growth and yield

Ali H. A. AL-Mansur

ANBAR JOURNAL OF AGRICULTURAL SCIENCES, 2011, Volume 9, Issue 2, Pages 286-293

Abstract
The present study was carried out to regulate the microbial activity in soil environment to be suitable for soybean so as the increase the activity of rhizobia fertelizering forming nodules and increasing the microbial inoculum activity through providing plant nutrients.
A pot experiment was conducted by culturing soybean seeds (gesa-65)in sandy loam soil. Tow isolates of Bradyrhizobium japonicum and Pseudomonas puteida were used after evaluating their activities in the laboratory as co- inoculums in addition to the treatment of a single B. japonicum as well as a control treatment .
Results have showd the ability of two isolates to produce IAA and dissolve phosphate compounds. P. puteida was able to produce NH3 and HCN as well as sidrophores and inhibit the growth of F. oxysporum mycelium. The co- inoculums of the two isolates were efficient for activity the highest rate of plant growth 93%, and nodules formation estimated after month of plant growth 42 nodule 90% activity. Also, the two isolates improved the nutrient status N, P ( 20.3,210 mg/kg soil) They increase the microbial density that dissolves phosphates and nitrogen fixation in the plant environment (5.26 ,5.32 Log cfu/g).The activity of the two isolates in soil environment was increasing the dry weight of the roots, vegetables, number of nodules , yields( 22.12 ,74.32 ,80 , 85.3 / plant), P and N conteats and protein percentage 36.25%.

The presence of Pseudomonas spp. bacteria in soil irrigated with wastewater

Rand M. Abdalrahman; Hutaf A. Alsalim

ANBAR JOURNAL OF AGRICULTURAL SCIENCES, 2011, Volume 9, Issue 1, Pages 188-196

The effect of treated and untreated wastewater on Pseudomonas spp. bacteria was detected. Soil samples were collected from a cotton cultivated field that divided into five plots which irrigated with different irrigation water : regular irrigation water (W0), well water (W1), untreated wastewater (W2), and two ways treated wastewater (W3,W4). The numbers of Pseudomonas spp. bacteria were significantly high in soil irrigated with wastewater in general (W3, W2, W4 respectively). The differences between these treatments (W3, W2, W4) were highly significant compared to the soil irrigated with well water (W1) and the soil irrigate with regular irrigation water (W0), as the last two treatments (W0,W1) did not differ significantly from each other. The study showed the presence of Pseudomonas fluorescens bacteria in the soil irrigated with regular water and well water (W0, W1) and the absence of these bacteria in soil irrigated with waste water. The study also indicates the present of Pseudomonas aeruginosa bacteria in the soil irrigated with treated and untreated wastewater (W2,W3,W4) and in large numbers, in addition to their presence (in low numbers) in soil irrigated with regular water and well water (W0,W1). This clarifies the role of using wastewater in changing soil environment and its natural flora.

Effect of the aqueous extract of Hibiscus sabdariffa L. and Prosopis farcta on some antibiotics resistance relatively bacteria

H. K. Y. Al-Salman

ANBAR JOURNAL OF AGRICULTURAL SCIENCES, 2009, Volume 7, Issue 1, Pages 391-399

The aims of this study were to investigate the effect of aqueous extract of the calyx of Hibiscus sabdariffa L. and fruits of Prosopis farcta on some resistance bacteria isolated from urinary tract infected patients. These bacteria were:
Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, Staphylococcus aureus.
These bacteria were tested for their sensitivity against several antibiotics and against several concentration of extracts.
It was found that the aqueous extract contain inhibitory action more than antibiotics against the tested bacteria.
The inhibitory action on gram positive bacteria Staphylococcus aureus was mor than that against gram negative bacteria Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris.

Effect of Turnsoles Chrozophora tinctoria L. Extracts on Some Pathological Bacteria Types

Thaer A. Kader

ANBAR JOURNAL OF AGRICULTURAL SCIENCES, 2009, Volume 7, Issue 1, Pages 369-378

A laboratory experiment was performed at microbiology lab./ College of Science, Al-Anbar Univ. during 2005, to estimate the effect of ethanolic and water extracts of turnisoles stem and leaves on four endemic bacteria E.coli, S.aereus, Ps.aeroginesa and Ps.mirabilis. Treatment were distribute within factorial arrangement applied at CRD design with three replicates. Result show that 50 mg/ L × ethanolic extract × stem part was significantly superior in solubility diameters of 27.67 and 27.37 mm of E.coli and S. aerens respectively. Also, it is indicated that 50 mg/ L × ethanolic extract × leaves part significantly gave highest solubility diameters of 21.00 and 14.00 mm of Ps.aeroginesa and P. mirabilis, respectively.

The effect of the aqeous extract of the leaves of Achillea fragrantissima on some Bacteria

Hanaa A. Yaseen

ANBAR JOURNAL OF AGRICULTURAL SCIENCES, 2008, Volume 6, Issue 1, Pages 296-300

The aim of the study was to investigate the effect of the Aqueous extract of the leaves of Achillea fragrantissima were tested in vitro on six species of bacteria: Staphylococcus aureus, Escherichia coli, Salmonella typhi, Bacillus subtilis kebsiella pneumoni and Pseudomonas a eroginosae.
The results indicated that the aqueous extract has an inhibitory effect on most of the tested bacteria.
The inhibitory action on gram positive bacteria, Bacillus subtilis and Staphylococcus aureus. Was more than that against gram negative bacteria.

Aerobic cellulolytic Bacteria from some soils of Ramadi city

Muthana H. Housin

ANBAR JOURNAL OF AGRICULTURAL SCIENCES, 2005, Volume 3, Issue 1, Pages 285-290

The aim of the present study was to isolate and diagnose aerobic bacteria.which have cellulolytic activity and determine the extent of their spearding in Ramadi city.Atotal of 20 soil samples were collection from 6 different location in Ramadi city .Asuitable selective medium was used for isolation.Several biochemical and cultural tests were done for diagnosis.The study revealed the following:
1- The soils of Ramadi city contained different genera of aerobic cellulolytic bacteria in ratio of 55% of total isolated bacteria .
2-These bacteria belonged to the genera Pseudomonas ,Micrococcus and Enterococcus in ratios of 45,5 and 5%, respectively .