Abstract

This study was undertaken to identify a strain of cucumber mosaic virus infected bean Phaseolus vulgaris cv. Pinto. Symptoms on indicator plants and of serological tests (double immuno-diffusion & Immunostrip-ELISA) were used to justify this goal. Symptoms manifested on bean and indicator plants indicated the presence of a strain B of cucumber mosaic virus (B-CMV). Partial purification of the virus isolate from N. tabacum cv. Rustica was achieved by precipitating the virus by 50% (w/v) ammonium sulfate. The absorption ratio at 260/280 nm of the purified preparations was found to be 1.7. The yield of the purified virus was 220 mg of virus/kg of plant leaves. An antiserum for the isolate with a titer of 1/512 was obtained by 4 intramuscular injections of purified virus at 2.6 mg/ml in a rabbit. Precipitation lines between wells containing the antiserum of the virus and those containing purified virus or extracts from infected plants by double immuno-diffusion test on agarose gel were appeared.